Low multiplex IF

(< 9 markers)

FFPE Immunofluorescence Tyramide Signal Amplification (TSA IF)

ProtocolMarkerTissue speciesOrganInstrumentTotal protocol timeStatus
6-plex panelFoxP3humanTonsilLabSat46minValidated
6-plex panelPD-L1humanTonsilLabSat51minValidated
6-plex panelPD-1humanTonsilLabSat36minValidated
6-plex panelCD68humanTonsilLabSat24minValidated
6-plex panelCD8humanTonsilLabSat36minValidated
6-plex panelCKhumanTonsilLabSat36minValidated

 

 

FFPE Sequential Immunofluorescence (seqIF)

ProtocolMarkerTissue speciesOrganInstrumentTotal protocol timeStatus
4-plex panelCD11chumanTonsilLabSat37minTested
4-plex panelCD11bhumanTonsilLabSat37minTested
4-plex panelCD15humanTonsilLabSat21minTested
4-plex panelCD56humanTonsilLabSat21minTested

 

Validated: assay performance has been demonstrated with reproducibility and repeatability tests over multiple samples.
Tested: conditions have been tested to show the potential or fully optimized on one sample.