Automation of RNAscope™ assays in immuno-oncology research on LabSat®
LabSat® is an automated tissue stainer device, allowing high-quality fast multiplexing up to 6 markers within a few hours. Lunaphore’s core technology, the Fast Fluidic Exchange (FFeX), utilizes a microfluidic Staining Chip that delivers reagents sequentially onto a tissue sample. The staining chamber is filled almost instantaneously, preventing different areas of the tissue from being incubated unevenly hence providing a great degree of signal uniformity in an ultra-controlled environment, allowing more robust and reliable results. The system is pressurized and provides temperature control in order to finetune and optimize staining conditions. This is key for image processing purposes with digital pathology tools such as signal quantification.
As with other hybridization methods, performing the RNAscope assays requires incubation and amplification cycles. These are performed under temperature-controlled conditions. The LabSat® platform allows for automation of these important steps resulting in time savings and walk-away opportunities for scientists. During the single-plex proof-of-concept test on the platform, results were achieved in 6 hours and 30 minutes following a fully automated protocol, from pre-treatment to detection and counterstaining. The same experiment, when performed manually, required up to 8 hours.
Fast biomarker optimization on LabSat®
Biomarker discovery research in the field of immuno-oncology relies heavily on immunohistochemistry (IHC) assays, involving the execution of tailored protocols with a high number of steps, all of which are subject to optimization. This can be a lengthy process that can take days, weeks, and even months to perform, and requires a step-by-step approach to finetune protocol conditions such as assay temperature, antibody concentration, or incubation times, in order to achieve optimal results with minimal investment of resources.
Turnaround times of manual assays, even for single marker stainings, often require overnight incubations, and existing automated staining platforms, while faster, still require several hours to stain one marker and can appear to be very expensive when performed for high throughput, which turns these optimization phases into a long burdensome process.
The LabSat® platform is an automated tissue immunostainer device. The system is pressurized and provides precise temperature and reagent flow control in order to finetune and optimize conditions, allowing high-quality and fast multiplexing of up to 6 markers within a few hours, and can perform single-plex staining under 30 minutes. This short timeframe is particularly well suited to run fast protocols during assay optimization in just a few samples, instead of staining large batches of slides to save precious samples or reagents.
Fast and automated TSA-based multiplexed immunofluorescence on LabSat®
Multiplex immunofluorescence provides an edge to understand the coexpression and spatial distribution of multiple targets without compromising tissue integrity. Tyramide Signal Amplification (TSA) is utilized in immunostaining assays for its high sensitivity, being capable of detecting low expressed epitopes and generate precise and clear results.
The LabSat® staining platform allows high-quality fast multiplexing up to 6 markers + DAPI within a few hours.
The open system offered by LabSat® combines with the ultrasensitive TSA technique enables saving precious time and get reproducible results with high-quality.