Develop assays while preserving tissue morphology in multiplex immunofluorescence experiments with COMET™

Easy assay development for multiplex immunofluorescence

Date: June 9, 2022 | 3:00 PM CEST

Speaker: Emilie Peres, Application Development Engineer

Abstract: Spatial proteomics is the new frontier in life sciences that enables the understanding of complex intercellular interactions while preserving morphological information. Multiplex immunofluorescence assays enables the study of multiple biomarkers on the same tissue type. However, the development of multiplex assay is challenging, time and reagent consuming, and error prone. In this webinar, we will show how to take advantage of our fully automated and hyperplex platform, called COMET™, to overcome these challenges. COMET™ is based on a microfluidic-patented technology and performs sequential immunofluorescence. We will present how to develop hyperplex assays in a simple, flexible and reproducible manner. Using a guided workflow, protocols can be easily optimized and transferred to other tissue types, in a few steps on COMET™.

Hyperplex spatial biology without compromises: a focus on tissue morphology preservation, epitope stability and elution efficiency

Date: June 14, 2022 | 3:00 PM CEST

Speaker: Dr. Pino Bordignon, Team Leader Application Research

Abstract: Spatial immunofluorescence is a game-changer technique in the field of immune-oncology, neuroscience and infectious diseases. When developing multiplex immunofluorescence panels tissue preservation and efficient antibody removal are key factors to ensure signal specificity. COMET™ is based on a microfluidic technology and performs sequential immunofluorescence (seqIF™), with repetitive cycles of staining, imaging and elution. COMET™ performs fast and finely controlled antibody staining incubations without harsh tissue photobleaching or tissue ablation. In this webinar, we will highlight how to verify tissue integrity and demonstrate how COMET™ guarantees efficient  antibody elution while ensuring morphology preservation. We will also show how to assess epitope stability during seqIF™ assay and how COMET™ enables marker stability up to 20 cycles across different tissue types and species.

At the end of each webinar, there will be a live Q&A session, giving the opportunity to ask your questions to our speakers. Not available? Register anyway and access the webinars on demand.

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