SPYRE™ Signal Amplification Kit
Make every marker count
While most protein markers can be detected with standard indirect immunofluorescence methods, markers with low signal levels sometimes remain difficult to visualize. The SPYRE™ Signal Amplification, fully integrated with the COMET™ platform, amplifies the signal for low-expressed or hard-to-detect markers. It offers high-sensitivity detection you need without compromising on accuracy.
The SPYRE™ Signal Amplification advantages
Sensitive
Enhanced detection of challenging or low-expressed markers tailored to COMET™.
Quantifiable
Detect faint signals while preserving quantification accuracy, ensuring reliable results.
Flexible
Amplify the signal of your target in any cycle of your seqIF™ panel using any off-the-shelf primary antibody of mouse or rabbit origin. The amplified signal can be eluted for consecutive rounds of detection.
Seamless integration with COMET™
Based on sequential immunofluorescence (seqIF™) technology, the sequential layered amplification (seqLA™) method enhances the detection of secondary antibodies bound to primary antibodies by utilizing linker molecules.
SPYRE™ Signal Amplification
Composite image containing combination of seqIF™ and seqLA™ (amplified markers are identified with a *).
SPYRE™ Signal Amplification
Composite image containing combination of seqIF™ and seqLA™ (amplified markers are identified with a *).
Tunable amplification
A) Degree of amplification (DSA) is shown as a ratio of staining intensity compared to seqIF™.
B) Quantification as normalized mean intensity (NMI) in blue and signal-to-background ratio (SBR) in orange.
Tunable amplification
A) Degree of amplification (DSA) is shown as a ratio of staining intensity compared to seqIF™.
B) Quantification as normalized mean intensity (NMI) in blue and signal-to-background ratio (SBR) in orange.