Biomarkers & Precision Medicine

There is an urgent need for more robust methods to differentiate clinically relevant biomarkers, thereby improving precision medicine. Spatial biology is an emerging approach to studying the localization and spatial relationships of biomarkers. Lunaphore provides end-to-end solutions for spatial proteomics and spatial multiomics. We will present how the COMET™ platform performs sequential immunofluorescence (seqIF™) to obtain high-quality and reproducible data with standard off-the-shelf antibodies. We will explore our innovative multiomics application that combines RNAscope™ and seqIF™ to simultaneously identify RNA and protein targets on the same tissue section in a fully automated workflow. Additionally, we will present a novel multiplex imaging-based immunotherapy panel and a comprehensive analysis pipeline to characterize the spatial distribution and function of immune cells and their application for spatial biomarker detection in a cohort of immunotherapy-treated melanoma patients. Our guest speaker from CellCarta, a leading global contract research organization, will present how the COMET™ seqIF™ workflow brings the multiplexing capability of flow cytometry to the solid state of FFPE tissue with the added context of cellular spatial relationships. The integration of high-resolution image stacks and large data files into workflows traditionally optimized for low or single-plex immunofluorescence is challenging. We present an analytical framework to transform high-resolution multiplex immunofluorescence images into biologically meaningful insights, augmenting clinical trial data with exploratory insights into immune profiles, tumor microenvironment, and biomarker stratification.