Asia-Pacific Spatial Translational Research Alliance (ASTRA)

There is an urgent need for more robust methods to differentiate immunotherapy responders from non-responders. Spatial biology, which examines cellular localization and interactions, is crucial for understanding the tumor-immune microenvironment. Here, we show how Lunaphore provides end-to-end solutions for spatial proteomics and spatial multiomics. We will present how the COMET™ platform performs sequential immunofluorescence (seqIF™) to obtain high-quality and reproducible data with standard off-the-shelf antibodies. We will explore our innovative multiomics application that combines RNAscope™ and seqIF™ to simultaneously identify RNA and protein targets on the same tissue section in a fully automated workflow. Additionally, we will present a novel multiplex imaging-based immunotherapy panel and a comprehensive analysis pipeline to characterize the spatial distribution and function of immune cells and their application for spatial biomarker detection in a cohort of immunotherapy-treated melanoma patients. Our distinctive spatial biology approach empowers scientists to identify new molecular signatures and bolster the development of targeted therapies.