Develop assays while preserving tissue morphology in multiplex immunofluorescence experiments with COMET™

Spatial proteomics is the new frontier in life sciences that enables understanding of complex intercellular interactions while preserving morphological information. Multiplex immunofluorescence assays allow the study of multiple biomarkers on the same tissue type. However, the multiplex assay development is challenging, time and reagent-consuming, and error-prone. In this webinar, we will show how to use our fully automated and hyperplex platform, called COMET™, to overcome these challenges. COMET™ is based on a microfluidic-patented technology and performs sequential immunofluorescence. We will present how to develop hyperplex assays in a simple, flexible, and reproducible manner. Using a guided workflow, protocols can be easily optimized and transferred to other tissue types in a few steps on COMET™.

Spatial immunofluorescence is a game-changer technique in immune-oncology, neuroscience, and infectious diseases. When developing multiplex immunofluorescence panels, tissue preservation and efficient antibody removal is vital to ensure signal specificity. COMET™ is based on microfluidic technology and performs sequential immunofluorescence (seqIF™) with repetitive staining, imaging, and elution cycles. COMET™ performs fast and finely controlled antibody staining incubations without harsh tissue photobleaching or tissue ablation. This webinar will highlight verifying tissue integrity and demonstrate how COMET™ guarantees efficient antibody elution while ensuring morphology preservation. We will also show how to assess epitope stability during seqIF™ assay and how COMET™ enables marker stability up to 20 cycles across different tissue types and species.