Integrating hyperplex spatial proteomics into your research: why and how

A core facility’s perspective

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December 7 2021

On demand



To simultaneously visualize multiple markers within the same sample enables a more detailed view of cellular phenotypes and how they orient in space. This brings a more profound understanding of the function and interplay of different cell types in situ, which is of value for research related to many biological conditions. For most labs, accessing and applying emerging spatial methods for highly multiplexed detection of various types of molecules is not straightforward. The Spatial and Single Cell Biology (SSCB) platform at SciLifeLab offers several spatial omics methods, including advanced single-cell sequencing, spatial transcriptomics, in situ sequencing, spatial mass spectrometry, and spatial proteomics. In this talk, Dr. Stadler will present the possibilities for spatial omics as a service at Scilifelab, emphasizing the methods offered for spatial proteomics using highly multiplexed immunofluorescence. Her Spatial Proteomics team, as part of the SSCB platform, currently provides a service to analyze up to 30 proteins in the same tissue section using DNA-barcoded antibodies. Next year, the COMET™ instrument from Lunaphore will be fully integrated into the SSCB platform and offered as a service. COMET™ performs sequential indirect immunofluorescence to allow for multiplexed protein detection in 4 samples in parallel. In this talk, Dr. Stadler will show results obtained with COMET™ on several tissue types.

December 7, 2021


  • Service offered within the Spatial and Single Cell Biology platform at Scilifelab
  • Multiplexed spatial proteomics using sequential immunofluorescence
  • Building a multiplexed immunofluorescence panel with COMET™


Charlotte Stadler, Ph.D.

Charlotte Stadler, Ph.D.

Co-Director of the Spatial Biology Platform, Head of Spatial Proteomics


Charlotte Stadler is a co-director of the Spatial Biology Platform at Science for Life Laboratory. She is also the Head of the National Spatial Proteomics facility. She received her Ph.D. in 2012 within the framework of the Human Protein Atlas (HPA) project, with Prof. Emma Lundberg and Prof. Mathias Uhlén as her supervisors. During her PhD she established many of the protocols and pipelines for image based subcellular protein profiling that are used in the Human Protein Cell Atlas.
Today, her main focus is to develop and implement services and technologies that can be offered by the Spatial Proteomics Facility. Her internal research projects focus on the implementation of multiplexed imaging solutions into clinical practice but also on integrating different omics types for a more complete molecular understanding of the tissue sample biology. For more information: https://www.scilifelab.se/facilities/cell-profiling/ https://www.proteinatlas.org/