Lab Cluster: Institut du Thorax

Novel technologies that deliver single-cell data have shed new light on biological processes and brought many exciting findings into life science research. Easy access to omics technologies is needed to push the boundaries of research and fill the high demand for it. Lunaphore aims to fill that gap and bring spatial context to single-cell assays. Here, we will show how to generate reproducible spatial data with COMET™, a universal hyperplex solution for high-throughput spatial multimers.

The COMET™ platform performs sequential immunofluorescence (seqIF™) assays to detect 40 protein targets per automated run. Guided workflow optimization and flexibility of the system to use the off-shelf reagents allow fast and easy adoption of the methodology in every laboratory, enabling researchers to explore the cellular microenvironment from assay development to cohort analysis in a few weeks. Here, we will also present our new fully automated, spatial multiomics workflow that integrates the simultaneous detection of RNA, with RNAscope™ HiPlex, and protein, with seqIF™, on the same tissue section at the single-cell level.