October 9 - 10
Leuven
KU Leuven
See the brain like never before with COMET™
Join us at the New Horizons in Neurodegeneration conference to discover how COMET™ brings spatial multiomics to your neuroscience research. Explore COMET’s spatial multiomics workflow for the detection of RNA and protein markers in the same tissue section at true subcellular resolution. You can now explore neuronal circuits, glial interactions, and the complex brain microenvironment like never before.
Meet our expert scientists, see our fully automated, protease-free multiomics workflow in action, and learn how COMET™ makes scaling multiomics experiments straightforward at every stage of research. COMET™ helps you gain deeper insights to accelerate your research, whether you’re mapping healthy brain regions or studying disease progression.
Don’t miss the chance to meet us in person! Schedule a one-on-one meeting with our technical specialist team to discuss your projects, get recommendations, and see COMET™ in action.
Poster presentation
Poster #6
9-10 October
Exhibition Hall
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by amyloid-beta plaques, neurofibrillary tangles, neuronal loss, and cognitive impairment. While our understanding of AD has evolved, mapping the cellular and molecular interplay between neurodegeneration and neuroimmunology remains challenging. Recent innovations in spatial biology have enabled multiple advances through the detailed identification of cell lineages and intercellular interactions within the native architecture of many tissue types. In this study, we leveraged a spatial proteomics approach to map the cellular interactions underlying neurodegeneration in AD.
Formalin-fixed paraffin-embedded tissue sections of human AD and healthy brain samples were stained and imaged using the fully-automated sequential immunofluorescence (seqIF™) workflow on COMET™. Images were analyzed by HORIZON™ software to reveal in situ single-cell level features.
We designed a highplex proteomics panel to characterize the spatial localization of AD-associated protein aggregates, along with key cell populations such as neurons, astrocytes, microglia, and vasculature-associated cells in the same tissue section. Our analysis identified inflammatory activation of microglia and astrocytes in the proximity of amyloid-beta plaques. This spatial approach offers novel insights into the spatial relationships between neurodegenerative lesions and the neighboring cells, shedding light on the neuroimmune landscape of AD.
Speaker
Pedro Almeida, PhD
Application Development Scientist - Scientific Affairs
Lunaphore