Conference
SCSO 2025
Methods and applications for single-cell and spatial multi-omics
Book a meeting with usSeptember 9 - 12
Leuven Belgium
Visit our booth
Discover true spatial multiomics at SCSO 25
Join us and engage directly with our specialists to discover spatial multiomics on COMET™. Experience firsthand how you can simultaneously detect RNA and proteins within the same section, all at a subcellular level of detail.
Our protease-free, fully automated workflow enables multiomics scalability for all stages of research. Are you interested in elevating your spatial biology projects with our multiomics solutions? Contact us now to arrange a detailed discussion with our dedicated team.
Visit us at our booth
Instrument demo and raw data
Stop by the Lunaphore booth to meet our team, get a live COMET™ demonstration and freely analyze raw multiomics and proteomics datasets.
Arrange a meeting with our team of product specialists to learn more about our solutions and technology.
Presentation and Posters
Industry Talk
Tuesday 11 September
12:35 – 12:50
Abstract
Advances in spatial biology have enabled a deeper understanding of signaling networks and cellular interactions at the single-cell level. Techniques such as multiplexed immunofluorescence (mIF) and RNA in situ hybridization (ISH) enable the detection of multiple protein and RNA biomarkers. In complex tissues such as tumor microenvironments and neural tissues, precise information on cellular interconnections and signaling activity is crucial in understanding the biological processes involved in development and disease.
Here, we present a novel, fully automated spatial multiomics approach that integrates the RNAscope™ HiPlexPro assay and sequential immunofluorescence (seqIF™) protocols on the COMET™ platform for co-detection of RNA and protein targets on the same tissue section. The combined multiplexing capability of up to 12-plex RNA and 24-plex protein targets enabled a comprehensive understanding of complex and fragile tissues. This approach was applied to formalin-fixed paraffin-embedded human solid tumors and frozen mouse brain tissues, demonstrating the versatility and robustness of the technique.
Through this automated assay, RNAscope™ probes targeting biomarkers relevant to either immuno-oncology or neuroscience were combined with protein markers to profile multiple cell types and their microenvironment. RNA probes targeting key cytokines were combined with cell marker antibodies to map activated T cells and macrophages infiltrating the tumor. On brain samples, RNA probes targeting neurotransmitters, receptors, and other glial cells were combined with selected protein markers for in-depth profiling of neuronal and non-neuronal subpopulations.
Overall, our spatial multiomics approach demonstrated the potential and versatility of the combined RNAscope™ and seqIF™ protocols on the COMET™ platform in immuno-oncology and neuroscience and opened doors to new discoveries in studies of biomarker and drug development.
Keywords
Spatial multiomics, multiplex immunofluorescence, RNA in situ hybridation, automated workflow
Speaker
Pino Bordignon, Ph.D.
Team Leader Application Research
Lunaphore
Poster presentation
Poster ID 135
Author list
Pino Bordignon1, Alice Comberlato1, Arec Manoukian1, Daniel D. Azorín2, Paula Juričić1, Alix Faillétaz1, Anushka Dikshit3, Debia Wakhloo3, Emerald Doolittle3, Rose Delvillar3, Steve Zhou3, Li-Chong Wang3, Maithreyan Srinivasan3, Saska Brajkovic1
1Lunaphore Technologies, a Bio-Techne brand, Tolochenaz, Switzerland
2 Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland
3Advanced Cell Diagnostics, a Bio-Techne brand, Newark, California, USA
Abstract
The rise of spatial biology has transformed our ability to investigate tissue structures and cellular interactions at a single-cell resolution. Through techniques such as multiplexed immunofluorescence (mIF) and RNA in situ hybridization (ISH), it is possible to achieve a detailed mapping of key histological and cellular components along with their activation states. Combining those two technologies on the same tissue section could allow a better profiling of complex tissues like the mouse brain.
Here, using the COMET™ staining and imaging platform, RNAscope™ and sequential immunofluorescence (seqIF™) assays were integrated and fully automated for same-section RNA and protein detection on fixed-frozen mouse brain tissue slides. Through precise temperature control and gentle reagent distribution, sensitive frozen sections can now be used to visualize up to a 12-plex RNA and 24-plex protein panel. Using this newly developed multiomics assay, we designed a panel to deepen the understanding of normal brain function and architecture through mapping of molecular networks and by studying the interactions between major brain cell types (e.g., neurons, astrocytes, oligodendrocytes) and different immune cells (e.g., microglia and macrophages).
Our results highlight the compatibility of combined RNAscope™ and seqIF™ protocols with frozen sections while showing valuable insights into the interactions between the immune and nervous systems, baseline for neurodegenerative disorder studies.
Topic
Neuroimmunology (NEUR) – Interactions Between Immune and Nervous System
Keywords
Spatial – Multiomics – RNAscope – seqIF – COMET – Mouse – Brain
Speaker
Pino Bordignon, Ph.D.
Team Leader Application Research
Lunaphore
Industry Talk
Tuesday 11 September
12:35 – 12:50
Abstract
Advances in spatial biology have enabled a deeper understanding of signaling networks and cellular interactions at the single-cell level. Techniques such as multiplexed immunofluorescence (mIF) and RNA in situ hybridization (ISH) enable the detection of multiple protein and RNA biomarkers. In complex tissues such as tumor microenvironments and neural tissues, precise information on cellular interconnections and signaling activity is crucial in understanding the biological processes involved in development and disease.
Here, we present a novel, fully automated spatial multiomics approach that integrates the RNAscope™ HiPlexPro assay and sequential immunofluorescence (seqIF™) protocols on the COMET™ platform for co-detection of RNA and protein targets on the same tissue section. The combined multiplexing capability of up to 12-plex RNA and 24-plex protein targets enabled a comprehensive understanding of complex and fragile tissues. This approach was applied to formalin-fixed paraffin-embedded human solid tumors and frozen mouse brain tissues, demonstrating the versatility and robustness of the technique.
Through this automated assay, RNAscope™ probes targeting biomarkers relevant to either immuno-oncology or neuroscience were combined with protein markers to profile multiple cell types and their microenvironment. RNA probes targeting key cytokines were combined with cell marker antibodies to map activated T cells and macrophages infiltrating the tumor. On brain samples, RNA probes targeting neurotransmitters, receptors, and other glial cells were combined with selected protein markers for in-depth profiling of neuronal and non-neuronal subpopulations.
Overall, our spatial multiomics approach demonstrated the potential and versatility of the combined RNAscope™ and seqIF™ protocols on the COMET™ platform in immuno-oncology and neuroscience and opened doors to new discoveries in studies of biomarker and drug development.
Keywords
Spatial multiomics, multiplex immunofluorescence, RNA in situ hybridation, automated workflow
Speaker
Pino Bordignon, Ph.D.
Team Leader Application Research
Lunaphore
Poster presentation
Poster ID 135
Author list
Pino Bordignon1, Alice Comberlato1, Arec Manoukian1, Daniel D. Azorín2, Paula Juričić1, Alix Faillétaz1, Anushka Dikshit3, Debia Wakhloo3, Emerald Doolittle3, Rose Delvillar3, Steve Zhou3, Li-Chong Wang3, Maithreyan Srinivasan3, Saska Brajkovic1
1Lunaphore Technologies, a Bio-Techne brand, Tolochenaz, Switzerland
2 Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland
3Advanced Cell Diagnostics, a Bio-Techne brand, Newark, California, USA
Abstract
The rise of spatial biology has transformed our ability to investigate tissue structures and cellular interactions at a single-cell resolution. Through techniques such as multiplexed immunofluorescence (mIF) and RNA in situ hybridization (ISH), it is possible to achieve a detailed mapping of key histological and cellular components along with their activation states. Combining those two technologies on the same tissue section could allow a better profiling of complex tissues like the mouse brain.
Here, using the COMET™ staining and imaging platform, RNAscope™ and sequential immunofluorescence (seqIF™) assays were integrated and fully automated for same-section RNA and protein detection on fixed-frozen mouse brain tissue slides. Through precise temperature control and gentle reagent distribution, sensitive frozen sections can now be used to visualize up to a 12-plex RNA and 24-plex protein panel. Using this newly developed multiomics assay, we designed a panel to deepen the understanding of normal brain function and architecture through mapping of molecular networks and by studying the interactions between major brain cell types (e.g., neurons, astrocytes, oligodendrocytes) and different immune cells (e.g., microglia and macrophages).
Our results highlight the compatibility of combined RNAscope™ and seqIF™ protocols with frozen sections while showing valuable insights into the interactions between the immune and nervous systems, baseline for neurodegenerative disorder studies.
Topic
Neuroimmunology (NEUR) – Interactions Between Immune and Nervous System
Keywords
Spatial – Multiomics – RNAscope – seqIF – COMET – Mouse – Brain
Speaker
Pino Bordignon, Ph.D.
Team Leader Application Research
Lunaphore