November 9 - 11
Visit our booth #22
Join us in Lausanne for the SGPath Annual Meeting.
Meet our scientists on-site and discover our universal, end-to-end spatial biology solution, answering the needs of the scientific community from early discovery to late-stage translational and clinical research.
Our solutions allow you to minimize validation challenges and move fast from biomarker discovery to translational research.
Want to know more about our spatial biology solutions? Send us a message and secure a meeting with the team.
The scientific program has been developed around the theme “Pathology – Science in Motion” to highlight the dynamic changes taking place in our specialty that impact on diagnostic practice, as well as the rapid increase in data generation and knowledge enabled by novel technologies that advance analytical resolution and dimensionality.
9:30 - 10:30 AM
Room Turin AB - e-Poster session 2
Background: Deciphering the tissue architecture is emerging as a crucial step to better understand tissue biology and harness it in a future therapeutic intervention [PMID:32377572]. Newly developed protocols allow detection of dozens of biomarkers simultaneously [PMID:35714588;35132261]. However, fresh frozen sections (FS) analyses remain challenging as harsh procedures used in manual protocols are detrimental to tissue morphology and limit the use of this application [PMID:34811556]. Furthermore, manual protocols are laborious and time-consuming, allowing to process a limited number of samples. Here, we describe the capability to perform automated hyperplex assays for up to 32 biomarkers on FS as a cogent case combining staining quality with tissue preservation.
Methods: COMET™ platform automates sequential immunofluorescence (seqIF™) assays based on an iterative series of fast tissue staining, imaging, and antibody elution cycles. FS tissue of human and murine origins were fixed and permeabilized prior to staining protocols. Up to 32 biomarkers were detected on a single tissue slide. To assess the tissue morphology preservation, standard hematoxylin and eosin (H&E) staining was performed on FS freshly processed or post-seqIF™.
Results: Murine tissues were stained with panels of up to 6 proteins showing an accurate detection of both immune and organ-specific biomarkers. For a deep characterization of human lung cancer, 32 biomarkers were simultaneously detected on a single FS with optimal staining and a total procedure time of 23 hours. An H&E staining performed on the slide retrieved from the platform after the seqIF™ showed excellent preservation of the tissue architecture in comparison with an unprocessed FS slide.
Conclusion: This work demonstrates the feasibility of performing automated hyper-plex assays on a variety of delicate frozen samples with high-quality results. With this new toolbox, we aim to support and hasten discovery studies across multiple research fields by overcoming current limitations in spatial biology.
Joanna Kowal, Ph.D.
Senior Scientific Affairs Manager
12:15 - 12:30 PM
Inti Zlobec, Ph.D.
Head of the Translational Research Unit (TRU)
University of Bern, Switzerland