Spatial proteomics: a journey to adopt highly multiplexed immunohistochemistry technologies

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December 7 2021

On demand



Multiplex protein expression analysis in tissue using immunohistochemistry has rapidly evolved over the last decade from 2- and 3-plex assays to a field of new technologies enabling highly multiplexed assays of 40 or more targets. Knowing which spatial proteomic technologies would be most effective in your lab or group is difficult. In this presentation, we will walk through the value proposition for spatial proteomics, focusing on antibody- and immunohistochemistry-based spatial proteomics platforms, as well as technical review vignettes describing our lab’s experience evaluating and selecting the most appropriate media for our lab. We hope our experience can empower you on your journey into spatial proteomics.

December 7, 2021


  • Understanding of spatial proteomics platforms currently available.
  • Pros and cons of a select set of spatial proteomics platforms.
  • Decision criteria for selecting spatial proteomics platforms based on use case and workflow.


Alexander Klimowicz, PhD

Alexander Klimowicz, PhD

Principal Scientist - Immunology and Respiratory Discovery Research

Boehringer Ingelheim

Dr. Alex Klimowicz is a Principal Scientist in the Department of Immunology and Respiratory Discovery Research at Boehringer Ingelheim Pharmaceuticals, Inc. In this capacity he leads the Molecular Histopathology Group, implementing and applying cutting edge in situ techniques, whole slide imaging, and digital image analysis, to build target to disease linkage in human tissue specimens for projects and external collaborations across the Department. Alex holds a PhD in Molecular Biology, and has 10 years of experience in the fields of digital pathology and quantitative immunohistochemistry. Prior to moving to Boehringer Ingelheim, Alex was an Adjunct Research Assistant Professor in the Department of Oncology at the University of Calgary, where he led a core quantitative immunohistochemistry lab focused on cancer biomarker research.