Reagents

Tools for spatial biology research.

Robust and reliable spatial biology data requires trusted reagents.

The journey of scientific discovery and development can be an overwhelming challenge. Streamline and accelerate your research with Lunaphore’s complete product offering, which includes solutions to execute your workflow from A to Z.

We offer a wide selection of dedicated reagents for optimal results with our spatial biology platforms. With lot-to-lot consistency and strict quality control testing you can generate highly reproducible data. Choose from the selection below to ensure fast and scalable results.

Explore spatial context with the right tools

High-quality reagents with reproducible performance
Supported by extensive data
Ready-to-use

Importance of antibody validation

Antibody and assay validation ensures the reliability, accuracy, and reproducibility of multiplexed immunofluorescence results. The validation of antibodies is of utmost importance as it serves as a foundation for scientific progress in antibody-based assays. Antibody validation is the experimental evidence and documentation demonstrating a specific antibody’s suitability for its intended application or purpose1. Consequently, antibodies must exhibit specificity, sensitivity, and reproducibility. They should detect only the antigens of interest, even at low expression levels, while consistently performing across different batches of the same antibody clone. At Lunaphore, by establishing standardized protocols, sharing validated reagents and reference samples, and conducting inter-laboratory comparisons, we can improve the reproducibility of assays and facilitate result comparisons across various studies. 

Our antibody validation process

Antibody scouting 

Selecting an antibody clone that was previously well-referenced in the literature or tested in-house to assess its suitability for the sequential immunofluorescence (seqIF™) application on COMET™. 

Specificity verification 

Comparing staining performance of primary antibody candidates on COMET™ with positive organ. Assessing specific staining patterns by comparing positive and negative tissue. 

Sensitivity verification

Testing antibody on heterogenous tissue and confirming dynamic range across different cells and tissue regions. 

Cross-validation seqIF™-IHC

Performing seqIF™ with the selected antibody and IHC with the pathology-routine antibody on the same slide. Comparing staining pattern and intensity. 

Products

1 Weller MG. Ten Basic Rules of Antibody Validation. Anal Chem Insights. 2018; 13. doi: 10.1177/1177390118757462.