To increase the throughput of spatial profiling of the human central nervous system (CNS), a CNS-specific library of 100+ markers with an associated analysis platform was developed and validated using the Lunaphore COMET™. Optimizing antibody concentrations, cycle order, and standard operating procedures, while managing subsequent image analysis with background and autofluorescence subtraction, have allowed for the visualization of molecular signaling at the subcellular level. Our approach is project-driven, from onboarding projects to interrogation sessions with investigators, with the associated panels customized to each specific scientific question. This strategy can be used to validate data such as single-cell sequencing or for novel discovery and hypothesis generation.
