Spatial biology enables the interrogation of tissue architecture on a single-cell level. Transforming the rich image output into meaningful quantitative data is the next significant hurdle on the path to fully comprehend and interpret the intricate imaging data. HORIZON™ is an image analysis software tailored to COMET™ hyperplex immunofluorescence images. It seamlessly extends the COMET™ workflow, smoothly handling the large data sets. With its straightforward and user-friendly approach, this analysis tool supports cell-based analysis and provides researchers with a toolset for early and high-level assessment of data in a hyperplex immunofluorescent image. We will walk through image analysis workflow on HORIZON™ from a COMET™ image to biological insights.